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In the present study the effect of six different glycosaminoglycan-peptide conjugates on normal and rheumatoid fibroblast-populated collagen lattices has been examined. The six different compounds studied were (a) Hyal-4, -5, or -6 linked to a tripeptide (A1, S8, S10) of insulin-like growth factor I (IGF-I); (b) a peptide related to the IGF-I tripeptide sequence (A1, S8, S10); (c) the NH2-terminal peptide derived from the glycosylated LDL receptor; (d) a peptide corresponding to the structure found in the amino-terminal portion of human type-II procollagen; (e) a peptide derived from the carboxy-terminal region of human type-III procollagen; and (f) a similar peptide corresponding to the structural sequence of human type-I procollagen. Hyal-6-IGF-I and A1-S8-S10-IGF-I were the only two of the six compounds which had a significant inhibitory effect on normal and rheumatoid fibroblast-populated collagen lattices.Q:
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The immune system of fish is similar to that of mammals and amphibians; however, the regulatory mechanisms are distinct from those in mammals. Therefore, differentially expressed genes and their regulatory networks that may play a role in the immune system of fish need to be identified. In this study, we employed high-throughput Solexa sequencing technology to identify differentially expressed genes in the small intestine of seabream (Sparus aurata) after the seabream was challenged by Listeria monocytogenes. A total of 38,163,643 clean reads were obtained, among which 27,507,323 clean reads were mapped to the seabream genome. Upon challenge, the number of differentially expressed genes in the small intestine of seabream was 59 and 256 and the number of up- and down-regulated genes was 25 and 227, respectively. Of these differentially expressed genes, about 95% of them have no known functional descriptions. Among differentially expressed genes, a few gene families were identified to be significantly up-regulated following L. monocytogenes infection, such as cluster of differentiation 8, STAT6, Il1b and TNFα. These findings are useful for understanding the immune response of seabream to L. monocytogenes and provide a foundation for further exploration of the immune system of fish.Time to talk about myself
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